Training Offered

Microsurgical procedures in mice

Contact: Dr. Sequeira Lopez – msl7u@virginia.edu

  • Acute central blood pressure measurement.
  • Ambulatory radio telemetry blood pressure, temperature and ECG recordings (with DSI units).
  • Partial and total unilateral ureteral obstruction and release in neonatal and adult mice.
  • Complete unilateral ureteral obstruction in adult mice.
  • Transplantation of embryonic organs under the kidney capsule.
  • Nephrectomy.
  • Kidney ischemia reperfusion injury.
  • Bone marrow transplantation.

Cell differentiation

Contact: Dr. Sequeira Lopez – msl7u@virginia.edu

  • In vitro colony forming assays of hematopoietic cells.

Histology

We provide training protocols and assistance

Contact: Dr. Sequeira Lopez – msl7u@virginia.edu

  • Tissue harvesting
  • Tissue embedding
  • Paraffin and Frozen sections
  • Histochemical stainings (Hematoxylin and eosin, PAS, Mason’s Trichrome, Sirius Red, etc..)
  • Immunostaining
  • In situ hybridization
  • Clearing techniques
  • Microdissection
  • Analysis and quantification using Metamorph

Mouse breeding strategies

Contact: Dr. Sequeira Lopez – msl7u@virginia.edu


Cre Lox lineage tracing strategies

Contact: Dr. Sequeira Lopez – msl7u@virginia.edu


Advice for experimental planning using molecular approaches

Contact: Dr. Medrano – sm8z@virginia.edu

  • Cloning
  • CRISPR technology
  • Targeting design