[Jordan 1-17] Hosted by Paula Barrett.
Annette C. Dolphin PhD, FMedSci, Professor of Pharmacology, University College London.
The Dolphin group has expertise in the field of neuronal voltage-dependent calcium channels, and our current work has particular reference to the role of accessory subunits, Beta and Alpha-2-delta. They use a number of different techniques in our research: involving expression of constructs in primary cultures of neurons or in mammalian cell lines with or without endogenous channels, or Xenopus oocytes. They use patch-clamp electrophysiology to examine macroscopic calcium currents, and they also use a variety of different imaging techniques. They have made a number of antibodies to calcium channel subunits, and are using these, as well as epitope-tagged channels for studies of the molecular epitopes controlling the trafficking and processing of the different calcium channel subunits. They are also using fusion proteins for the biochemical study of calcium channel domain interactions using surface plasmon resonance. The yeast two hybrid technique is also being used to identify novel interactions.