The Carter Immunology Center (CIC) occupies most of the top two floors of the recently built Carter-Harrison Research Building (MR6). The CIC research space adjoins other buildings in the UVA Health System, so the sense of a scientific community is very strong. These modern laboratory facilities provide an outstanding environment for conducting cutting edge research in immunology. The CIC floorplan comprises about 40,000 ft. sq. of space – allowing for a large administrative area, extensive common equipment rooms, laboratory space for ten investigators.The immunology students and post-docs have access to comprehensive research support services and equipment. The CIC maintains extensive shared instrumentation to assist Center Members in their research. The CIC administrative offices offer extensive services (e.g. help with visas, help with logistics, a poster printer).In close proximity to the CIC there can be found an array of strong technical core facilities. Most important among these cores is the UVA Flow Core which provides great training opportunities, an extensive array of flow cytometers, but also cell sorters, Imagestream, Luminex and CyToF (Cytometry by Time of Flight), technology that is truly cutting edge.
The newly-renovated Claude Moore Health Sciences Library, houses a huge collection of books and 2,900 journals, many of which are also available free on-line in full-text form to UVa students and faculty. In addition, the Carter Immunology Center library also hosts a specific collection of immunology-related journals. The Information Technology Services (ITS) provides expert assistance on computer-related issues from data acquisition and imaging to broad area networking and bioinformatics.
Other Core Facilities
The School of Medicine supports several state-of-the-art core facilities that are available to investigators on a fee-for-service basis. These facilities are administered by Jay W. Fox, Ph.D., Director, Research Infrastructure, and an advisory committee. Each core is overseen by a faculty committee; the SOM Research Advisory Committee (RAC) recommends annual subsidies to the Dean.
- Advanced Microscopy Facility. The Advanced Microscopy Core (AMC) provided expertise, training and instrumentation associated with confocal and electron microscopy (TEM and SEM). Sample preparation and operation of core instrumentation can be performed by core staff or trained users.
- Molecular Electron Microscopy Core. The MEMC specializes in electron cryomicroscopy (cryoEM), particularly for structure determination of biological complexes and proteins. It houses three electron microscopes (120kV Spirit, 200kV F20, and 300kV Titan Krios) that are available to trained users, or projects developed with core staff. The Titan Krios is also equipped with a direct electron detector for high-resolution data analysis.
- Biomolecular Analysis Facility. The Biomolecular Analysis Facility provides a range of services for analyzing peptides and proteins using instruments and expertise not available to individual laboratories. The Mass Spectrometry/Proteomics area supports experimentation in protein identification, post-translational modification analysis, binding partner/complex mixture analysis and quantitative mass spectrometry utilizing iTRAQ and SILAC approaches.
- BioNMR Core (Contact Jeff Ellena, Laboratory Director or Dr. John Bushweller, Faculty Director). The BioNMR Core has six NMR spectrometers (600MHz and 800MHz) for use by trained investigators or core staff. Expert consultation in molecular structural elucidation and molecular dynamics is available.
- Biorepository and Tissue Research Facility. This resource provides prospective and banked human tissue procurement; processing and storage of all types of biospecimens including blood, urine and tissues; enzymatic dissociation and freezing of tissue samples; and extraction of nucleic acids and proteins from tissues. Laser microdissection, immunohistochemistry and tissue microarray production are also available.
- Flow Cytometry Core Facility. The core maintains a variety of cell sorters and analyzers for state of the art cellular characterization. Expert consultation is available for experimental design and instrumentation is available for use by trained investigators or experiments can be performed by the staff. Several training/educational programs are offered throughout the year for investigators, students and staff.
- Genetically Engineered Murine Model Core. The range of services offered by the GTTF include genetic engineering of mutant mice; genetic analysis of mutant mouse strains and cryopreservation of mutant lines. The facility offers a yearly educational workshop to train investigators and staff in technologies associated with genetic manipulation of mice.
- Genome Analysis & Technology Core. The GATC mission is to serve as a scientific resource and biotechnology hub enhancing the scope and quality of basic and translational research at UVA, and to provide customer satisfaction, collaboration and quality control. The goal of the core staff is to enable high throughput (Next Generation) sequencing projects for both novice and experienced users alike, by providing timely assistance in all facets of experimentation from project design/execution to data analysis/reporting.
- Antibody Engineering and Technology Core. This core has current technologies to discover and engineer antibodies in multiple formats for specific needs. The core also makes monoclonal antibodies using hybridomas and combinatorial antibody libraries.
- Molecular Imaging Core. The aim of the core is to provide noninvasive monitoring of biological processes in vivo using a variety of anatomical and functional imaging modalities. Additionally development of targeted imaging agents is available. Instrumentation for the modalities of X-Ray CT, MRI, PET, SPECT and bioluminescence are available. A cyclotron generates radioisotopes for PET imaging and is associated with a radiochemistry laboratory which synthesizes imaging agents.
- MolMart. This facility provides common molecular biological reagents and supplies for purchase. Reagent pick-up at the site is also available.
- Research Histology Core. The Research Histology Core offers high quality, high throughput histology services including routine embedding, sectioning and H and E and special staining protocols. This core works in close affiliation with the BTRF to coordinate efforts to support investigators.
- Tissue Culture Core. Services provided by the Tissue Culture Core include cell line culture, propagation and storage as well as primary cell culture and baculovirus production. A variety of cell culture reagents are available in the laboratory for investigator purchase.